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Option 1: This statement is not the correct definition of bio-fortification. Bio-fortification refers to increasing the nutritional value of crops, not the nutrient quality of the soil.
Option 2: This is the correct definition of bio-fortification. It involves breeding crops to increase their nutritional values, such as increasing the levels of vitamins or minerals in the crop.
Option 3: This statement is not the correct definition of bio-fortification. It refers to the increase of organo-metallic compounds in an organism through energy flow in an ecosystem, which is not related to bio-fortification.
Option 4: This statement is not the correct definition of bio-fortification. It refers to the adoption of genetic variable plants for breeding high yielding varieties, which is not specific to increasing the nutritional value of crops.
Therefore, the correct answer is option 2, which defines bio-fortification as breeding crops to increase their nutritional values.