The correct sequence of various levels of organization of the Biosphere is option 4: Organism - Population - Ecosystem - Biosphere.

At the lowest level of organization, an organism refers to an individual living entity, such as a plant or an animal. The next level is population, which refers to a group of organisms of the same species living in the same area and able to interbreed. Moving up, an ecosystem is a collection of different populations living in a particular area and the physical environment they interact with.

Finally, the highest level of organization is the biosphere, which includes all of the ecosystems on Earth and encompasses the entire living world. It comprises all the plants, animals, and microorganisms on Earth, along with their physical environment.

This sequence is logical as it starts from the smallest unit (organism) and builds up to the largest and most inclusive level (biosphere). It shows how individual organisms form populations, which interact within an ecosystem, and ultimately contribute to the functioning of the entire biosphere.